KMID : 1011620190350060672
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Korean Journal of Food and Cookey Science 2019 Volume.35 No. 6 p.672 ~ p.676
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Changes in Protein Content and Thermal Denaturation Property of White-Spotted Flower Chafer (Protaetia brevitarsis) Larvae Powders Prepared by Different Pretreatment Methods
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Ham Youn-Kyung
Song Dong-Heon Noh Sin-Woo Kim Hyun-Wook
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Abstract
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Purpose: The objective of this study was to evaluate changes in protein content and thermal denaturation properties of white-spotted flower chafer (Protaetia brevitarsis) larvae, powdered using different pretreatment methods.
Methods: White-spotted flower chafer larvae powders were prepared by applying the following three methods: oven-dried larvae powder (OLP), oven-dried at 100¨¬C for 16 hr without any pretreatment process; frozen and oven-dried powder (FOLP), frozen at -20¨¬C for 24 hr and oven-dried at 100¨¬C for 16 hr; and frozen and freeze-dried larvae powder (FFLP), frozen at -70¨¬C for 24 hr and freeze-dried (-45¨¬C, 0 m Torr) for 5 days.
Results: No differences were observed in the moisture content and crude ash content among the larvae powders (p>0.05). However, FFLP exhibited lower crude protein and crude fat contents compared to OLP and FOLP treatments (p<0.05). The order of protein solubility in the white-spotted flower chafer larvae powder was determined to be FFLP (95.66%) > FOLP (76.20%) > OLP (68.71%) (p<0.05). Moreover, the results from differential scanning calorimetry (DSC) analysis showed that FFLP had significantly lower onset and peak temperatures, but higher ¥ÄH than OLP and FOLP treatments. The results from protein SDS-PAGE indicate that although larvae powders prepared with the oven-drying process (OLP and FOLP treatments) present decreased intensity of protein bands (< 50 kDa), a new protein band (70~100 kDa) was also observed.
Conclusion: The results of this current study indicate that freeze-drying could be an excellent pre-treatment method to produce white-spotted chafer larvae powder with minimizing the protein denaturation. Moreover, if oven-drying conditions (time and temperature) are fixed, freezing prior to oven-drying reduces the extent of protein denaturation in white-spotted flower chafer larvae powder as compared to direct oven-drying of the raw ingredient.
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KEYWORD
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DSC, edible insect, thermal denaturation, protein solubility
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